The NRG ErbB the cardiovascular effects

The role of experiments in heart development, the NRGs, ErbB2 and ErbB4 an important role in the development of the nervous system and the heart. NRG ErbB promote the proliferation of cardiomyocytes, hypertrophic growth inhibition of cardiomyocyte apoptosis and promote cell survival. GGF2 to promote embryonic rat neonatal rat cardiac myocytes DNA synthesis, and promote an increase in the number of myocardial cells. A net increase of the number of functional myocardial cells in the embryonic heart development process, related with the proliferation of myocardial cells, is still associated with myocardial cell survival. Study also observed that nearly 25% of the cells in serum-free matrix in primary cultures of neonatal rat cardiac myocytes number 4d died; contrary, a 30% increase in the number of cells added GGF2 GGF2 promote survival of cardiomyocytes in vitro . Expression the no function NRGs and ErbBs rats died of heart and nervous system abnormalities in the uterus. NRG1, ErbB2, ErbB3, ErbB4 is no function of the mutated gene experiments showed that embryonic mice, mutations in several other genes outside than ErbB3 and development 10. 5d, due to poor cardiac differentiation, lack of ventricular trabeculae and endocardial cushion while resulting in the death of the embryo. Hertig application NRG1 and insulin-like growth factor diffuses into the chambers of the heart found, NRG1 can promote the formation of trabecular ventricular wall, and insulin-like growth factor 1 had no effect. In addition, NRG1 and insulin-like growth factor 1 synergistically induced cardiomyocyte DNA synthesis, and promote the growth of the ventricular tight zone and AV cushion after two heart chamber development and maturation of the key. Prompt the NRG1 and insulin-like growth factor 1 interaction occurs affect the morphology of the heart chamber. Zhao et GGF2 with other pro-mitogen not identical, in serum-free low density alkylene fusion myocardial cell culture, GGF2 mechanism to promote the survival of neonatal and adult cardiomyocytes, in part is due to inhibition of apoptosis.


The cardiovascular system NRG ErbB widely expressed in the cardiovascular system

The cardiovascular system NRG ErbB widely expressed in the cardiovascular system, but differences in their distribution in different tissues. Mainly distributed in the intima of developing ventricular NRG1mRNA, NRG2 mRNA is mainly distributed in the intima of developing atrial NDF (neu 1mR NA) expression in rat coronary microvascular endothelial cells in cardiac development and pathological hypertension are induced cardiac hypertrophy, myocardial cells and neighboring endothelial cells highly expressed NRGs. While rat embryonic development of the mid-ErbB2, ErbB3 and ErbB4 expression After ErbB3 expression was significantly down-regulated. ErbB2, ErbB4 distribution in atrial and ventricular myocardium, ErbB3 distributed in the mesenchymal cells in the endocardial cushion and Cordis ball. In human umbilical vein endothelial cells detected immune response kind of ErbB2, ErbB3 and ErbB4

Nervous system and other tissues in the nervous system of the neurons, astrocytes, oligodendrocytes, glial cells and Schwann cells receptor expression are ErbBs. Retinal neurons, breast cancer cells have NRGs distribution. Peripheral listen ganglion and vestibular ganglion ARIR highly expressed. NRG4 only expressed in mature cells of the pancreas, which showed low levels of expression in muscle cells.

neuromodulation B Receptor Agonist (NMBR) gestational age of pregnant rats and explore its impact mechanism

Observed neuromodulation B Receptor Agonist (NMBR) gestational age of pregnant rats and explore its impact mechanism. The establishment of accurate gestational age of pregnant mice model, randomized (n = 10). Pregnancy 18d2 6: 00pm, respectively, intraperitoneal injection of NMB (30,90,150 g kg-1), oxytocin (50mIU kg-1) and the volume of solvent, continuous 2d observation of pregnant rats of gestational age and birth interval. Pregnancy 18d6 8,10,12: 00am were given the above treatment, 4h after the last administration to take the pregnant rat uterine smooth muscle tissue, the use NoShift transcription factor analysis to detect the DNA binding activity of NF BP65, semi-quantitative RT PCR and Western blot detection of HSP70 of IL 6mRNA and protein expression. Results 150 gkg-1NMBR group gestational age was significantly shorter than in the control group and low dose group (P <0 05); NF BP65DNA combination of activity, HSP70, and of IL 6mRNA expression level was significantly higher (P <0 05). The conclusion NMBR agonists may be pregnant rat uterine smooth muscle NF the BP65 HSP70/IL 6 pathway to shorten gestational age.

Neuromodulation B Receptor (neuromedinB receptor, NMBR) is a mammalian bombesin (bombesin, BB) receptor family members, showed a significant difference in expression in uterine smooth muscle before and after the labor. The NMBR role in the onset of labor and the mechanism is unclear, but studies have shown that the NMBR upstream gene with neuroendocrine function, with G-protein coupled receptor (Gprotein coupled re ceptor, GPCR) structure of the downstream gene nuclear factor B (nuclearfactor {kappa} B NFB) is the hub of transcriptional activation of the cells within a variety of ways, and modulation of cytokines, the production of stress proteins, suggesting that the NMBR the basis of good contractions of drug targets. In this paper, the effects of different doses of NMBR agonists of pregnant

Mouse gestational age and birth interval, clear NMBR role in the delivery start; NFBP65DNA pregnant uterus smooth muscle cells through its binding activity of heat shock protein 70 (heat shockpro tein70, HSP70) and interleukin 6 (of interleukin 6, IL 6) mRNA and protein expression to explore the mechanism of the the NMBR influence of gestational age.

NRGs by six different domains: the amino-terminal region of the immunoglobulin-like domain

Neuromodulation prime NRGs by six different domains: the amino-terminal region of the immunoglobulin-like domain, EGF-like domain, the juxtamembrane sequence of the transmembrane sequence and cytoplasmic domain. The NRGs existence of different forms of soluble and membrane chimeric. Most of the the NRGs First synthesis and membrane-bound precursor proN RGs proNRGs followed to produce soluble NRGs, and secretion of this form. NRGs may play a role in cell paracrine autocrine form.

ErbBs receptors ErbBs receptors membrane receptor, its structure consists of an extracellular binding site domain, a transmembrane domain, a tyrosine kinase domain and a C-terminal domain. The ErbBs receptor subtypes may form homo-or heterodimer, dimer functions and constitute a dimer receptor subtypes to stimulate the ligand and is activated in the intracellular signal transduction pathways related